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KMID : 0903520040470040384
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2004 Volume.47 No. 4 p.384 ~ p.389
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Antioxidant Action of Reaction Mixtures of Gums Hydrolysates and Urea Derivatives
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Kim Sang-Woo
Park Gwi-Gun
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Abstract
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The purified β-mannanase hydrolyzed various gums to mannose, β-1,4-mannobiose, Gal3Man4, and D.P 7 of galactosyl mannooligosaccharide, and isolated from the enzymatic hydrolysate for 24 hrs reaction by activated carbon column chromatography and Sephadex G-25 column chromatography. For the elucidate of antioxidant action of β-1,4-mannobiose, Gal3Man4 and DP 7 of galactosyl mannooligosaccharide and urea derivatives, coloration, reducing power, antioxidant activity and DPPH test were accomplished. The coloration was high at reaction mixture of β-1,4-mannobiose, Gal3Man4, D.P 7 and urea. TLC of reaction mixture of β-1,4-mannobiose, Gal3Man4, D.P 7 and ureas showed new reaction products, respectively. but except reaction mixture of β-1,4-mannobiose and urea. The reducing power was high at reaction mixture of β-1,4-mannobiose, Gal3Man4 D.P 7 and phenylthiourea. The reaction mixture of β-1,4-mannobiose, Gal3Man4 D.P 7 and thiourea showed similar radical scavenging activities on DPPH to activity of AsA. The reaction mixture of β-1,4-mannobiose, Gal3Man4 D.P 7 and thiourea, phenythiolurea shown strong antioxidative activites on the oxidation of linoneic acid.
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KEYWORD
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antioxidant action, galactosyl mannooligosaccharides, β-1, 4-mannobiose
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